Differential Staining for Conjunctival Impression Cytology

S.C. Perera; A.P. Cullen

doi:10.65636/cjo.v52i3.7078

Authors

S.C. Perera
A.P. Cullen

DOI:

https://doi.org/10.65636/cjo.v52i3.7078

Keywords:

conjunctival disease, trauma, differential staining, cytology

Abstract

Conjunctival disease or trauma results in variability in cell type and numbers as determined by impression cytology. Differenntial staining is effective in facilitating identification of specific cell changes. Differential staining on conjunctival impressions revealed surface epithelial changes and the presence of eosinophils following exposure to ultraviolet B.

References

1. Egben PR, Lauber S, Maurice DM. A simple conjunctival biopsy. Am J. Ophthal 1977, 84(6):798-801. DOI: https://doi.org/10.1016/0002-9394(77)90499-8

2. Wittpennn JR, Tseng SCG, Sommer A. Detection of early xerophthalmia by impression cytology. Arch Ophthal 1986, 104:237-239. DOI: https://doi.org/10.1001/archopht.1986.01050140091027

3. Hatchell DL, Sommer A. Detection of ocular surface abnormalities in experimental vitamin A deficiency. Arch Ophthalmol 1984, 102: 1389-1393. DOI: https://doi.org/10.1001/archopht.1984.01040031131040

4. Tseng SCG. Staging of conjunctival squamous metaplasia by impression cytology. Ophthalmol 1985. 92(6):728-733. DOI: https://doi.org/10.1016/S0161-6420(85)33967-2

5. Adams, GGW, Dilly PN. fmpression cytology as a method of monitoring ocular disease. Proceedings of the International Society for Eye Research. volume 5, 1988. p. 133.

6. Ratnakar KS, Goswamy V, Agarwal LP. Mast cells and pterygium. Acta Ophthalmol 1976, 54: 363:368. DOI: https://doi.org/10.1111/j.1755-3768.1976.tb01265.x

7. Hill JC, Maske R. Pathogenesis of pterygium. Eye 1989, 3:218:226. DOI: https://doi.org/10.1038/eye.1989.31